GSM5600646: EGR1 CHIP-seq CD34 HSPC-3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EGR1
Cell type
Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD34+ Hematopoietic and Stem Progenitor Cell
shRNA
NA
days post shrna infection
NA
cell type
CD34+ Hematopoietic and Stem Progenitor Cell
chip antibody
anti-EGR1 588 (Santa Cruz sc-110)
Sequenced DNA Library
library_name
GSM5600646
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was isolated from CD34+ HSPCs with Trizol. ChIP samples were generated from isolated HSPC nuceli using the EGR1 specific antibody 588 from Santa Cruz (sc-110) RNA libraries were constructed using Illumina's TruSeq Stranded mRNA kit. DNA libraries for ChIP seq were constructed using Tecan's Ovation DNA library preparation kit.