Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EGR1

Cell type

Cell type Class
Blood
Cell type
CD34 Hematopoietic stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD34+ Hematopoietic and Stem Progenitor Cell
shRNA
NA
days post shrna infection
NA
cell type
CD34+ Hematopoietic and Stem Progenitor Cell
chip antibody
anti-EGR1 588 (Santa Cruz sc-110)

Sequenced DNA Library

library_name
GSM5600646
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was isolated from CD34+ HSPCs with Trizol. ChIP samples were generated from isolated HSPC nuceli using the EGR1 specific antibody 588 from Santa Cruz (sc-110) RNA libraries were constructed using Illumina's TruSeq Stranded mRNA kit. DNA libraries for ChIP seq were constructed using Tecan's Ovation DNA library preparation kit.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
70120114
Reads aligned (%)
97.4
Duplicates removed (%)
16.9
Number of peaks
5381 (qval < 1E-05)

hg19

Number of total reads
70120114
Reads aligned (%)
97.2
Duplicates removed (%)
17.3
Number of peaks
5520 (qval < 1E-05)

Base call quality data from DBCLS SRA